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Nitrosamine Risk Assessments

The understanding of the process chemistry with respect to the risk of API containing N-Nitrosamines as impurities is essential to ensure patient safety and meet regulatory requirements. Sai Life Sciences has a robust end to end process to assess risk and support customers in the development of control strategies.

Nitrosamine Risk Assessments
Mitigate N-Nitrosamine formation risks

Highlights

  • Robust risk assessment process as part of the quotation, manufacture and lifecycle management stages.
  • Vendor assessment process covering raw materials and starting materials.
  • Ongoing risk assessment process to mitigate any risk of N-Nitrosamine contamination as a result of the utilisation of process equipment.
  • Expertise in process chemistry to understand and mitigate the risk of N-Nitrosamine formation.
  • Expertise in the utilisation of in-silico purge calculations and spiking/purge studies to support control strategy development.
  • State of the art analytical instrumentation and expertise capable of meeting regulatory requirements for the control of N-Nitrosamines.
  • Capability to carry out Mutagenicity testing (Non-GLP Studies such as; Ames, In Vitro Chromosome Aberration Assay, and In Vivo Micronucleus Test)

Achievements

  • 100+ projects (RM, KSM, intermediates and API) were assessed and evaluated by Expert Panel Team at Sai and provided N-Nitrosamine Assessment reports for regulatory requirement as per client request
  • Synthetic route designs which minimise the risk of N-nitrosamine formation
  • Utilisation of purge calculations to inform the approach to regulatory control strategies.

State of the art analytical Instrumentation

  • Sciex LC-MS/MS ion trap
  • Shimadzu GC-MS/MS ion trap
  • Methodology has been established for a number of the N-Nitrosamines with ADIs approved by the regulatory authorities with a limit of quantification of 10ppb
  • Flexibility to extend the approach to other N-Nitrosamines of interest